Mycobacterium tuberculosis ag85ab chimeric dna vaccine was thus. Dna vaccines is a major updated and enhancement of the first edition. Therapeutic effect of dna vaccines given during chemotherapy on reactivation in a lowdose aerosol infection model. The mechanisms of ag85a dna vaccine activates rna sensors. Pdf a novel dna vaccine expressing the ag85aha2 fusion. Mycobacterium tuberculosis ag85ab chimeric dna vaccine was thus constructed by inserting the sequence encoding amino acids 125282 of ag85b protein into nucleotide 430435 endonuclease enzyme acc i site of ag85a dna vaccine. Original article polyethylenimine modified nanoparticle. It is also critical to evaluate the role of mammalian glycosylation in altering cellular immunity. Influence of the formulation characteristics on immune responses by johanna poecheim 1, christophe barnierquer 2, nicolas collin 2 and gerrit borchard 1. It elevated the immunogenicity and protective as well as therapeutic effects of the hsp65 dna vaccine against tb in mice. The immune response of hybrid striped bass morone saxatilis x morone chrysops following oral delivery of a dna vaccine containing mycobacterium marinum ag85a plasmid in either. Construction of a dna vaccine based on the mycobacterium tuberculosis ag85ampt64 fusion gene and evaluation of its immunogenicity.
Immunogenicity of a dna vaccine encoding ag85atb10. August 2007, dna vaccination against sclerosis was reported as being effective. Chapter 3 immunogenic and protective effects of a dna vaccine. Dna vaccines expressing the individual antigens were either beneficial or at least. A novel dna vaccine vector encoding the mycobacterium tuberculosis secreted antigen ag85a fused with the influenza a virus iav ha2 protein epitopes, pegfp ag85a sha2 pag85asha2, was designed to provide protection against influenza. Tuberculosis dna vaccine encoding ag85a is immunogenic and. Different ratios of pdna with cationic trimethyl chitosan tmc nanoparticles were characterized for their morphology and physicochemical characteristics size. The study on dna vaccines first began in the 1990s, when the plasmid dna is injected into the skin or muscle was reported to induce antibody responses to antigens.
Use of therapeutic dna vaccines is a promising strategy against tb. Request pdf immunogenicity and therapeutic effects of ag85ab chimeric dna vaccine in mice infected with mycobacterium tuberculosis the situation of tuberculosis tb is very severe in china. In addition, these antigens represent vaccine components that were tested in early human clinical trials. Dna vaccination is the method of choice for the induction of protective th1 type immune responses with the ag85a tuberculosis dna vaccine. Accordingly, dna encoding ag85a is currently contemplated as a promising dna vaccine candidate against mtb due to its ability to stimulate humoral and cellmediated immune responses in mice, as well as protection in mice against mtb challenge. Another method to improve dna vaccine immunogenicity is the inclusion of additional plasmids, or additional inserts in the same plasmid, encoding molecular adjuvants. This model has been instructive, in that in this system the mycobacterial protein is made by the mammalian host cell rather than by the organism.
Protection against mycobacterium tuberculosis challenge in mice by dna vaccine ag85a. Effects of the fusion design and immunization route on the. Construction of a dna vaccine based on the mycobacterium tuberculosis ag85a mpt64 fusion gene and evaluation of its immunogenicity. When the dna vaccine is administered to a patient, the machinery in their cells makes a viral or bacterial protein which their immune system. Indeed, vaccination of c57bl6 mice with plasmid dna encoding ag85a and ag85b can stimulate strong humoral and cellmediated immune responses and confer significant protection against aerosol or intravenous challenge with live mtb h37rv. The aim of the present work was to investigate the in. Sep 12, 2016 the aim of the present work was to investigate the influence of formulating a dna vaccine encoding ag85a with tmc nanoparticles, on the immune responses generated in mice. Tuberculosis remains a major health problem affecting millions of people worldwide 5. In summary, these findings open new perspectives in the area of mucosal dna vaccine, against specific pathogens using a. A novel dna vaccine expressing the ag85aha2 fusion protein. Mar 01, 20 use of therapeutic dna vaccines is a promising strategy against tb. Jan 21, 2020 it is also critical to evaluate the role of mammalian glycosylation in altering cellular immunity. Dna vaccination is a technique for protecting against disease by injection with genetically engineered plasmid containing the dna sequence encoding the antigens against which an immune response is sought so cells directly produce an antibody, producing a protective immunological response.
Dna vaccines have potential advantages over conventional vaccines, including the ability to induce a. Pdf ag85aesat6 chimeric dna vaccine induces an adverse. Construction and immunogenicity of a39 dna vaccine against. Noninfected mice immunized with rv2190c dna or ag85a dna showed higher levels of interferongamma ifn.
Previous studies have demonstrated that dna vaccines exert profound therapeutic effects in mice with tb 5,6. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on dna vaccine design and enhancement, delivery systems, production, purification, and quality. Designing and construction a dna vaccine encoding the. In this study, we developed a dna vaccine encoding the ag85aha2 fusion protein with the aim of inducing protection against both iav and s. Human trials testing dna vaccines against influenza virus, malaria, hepatitis b virus, hiv, herpes simplex virus, colon cancer, and cutaneous tcell. Immunogenicity and therapeutic effects of a mycobacterium tuberculosis rv2190c dna vaccine in mice yan liang1, xiaoyan zhang1,2, xuejuan bai1, li xiao1, xiaomei wang1, junxian zhang1, yourong yang1, jinying song1, lan wang1 and xueqiong wu1 abstract background. Dna vaccines encoding the 32 000 mw mycolyltransferase ag85a and the 40 000 mw phosphatebinding protein psts3 can elicit protective immune responses against experimental infection with mycobacterium tuberculosis in c57bl6 mice. Tuberculosis tb is one of most important chronic infectious diseases caused by mycobacterium tuberculosis and remains a major global health problem. Tuberculosis tb is a major global public health problem.
Figure 1 therapeutic effect of dna vaccines given during chemotherapy on reactivation in a lowdose aerosol infection model. In the present study, a chimeric dna vaccine was constructed that. Antibody production igg and siga antiag85a was also significantly increased in bronchoalveolar lavage, as well as in the serum of mice. Immunogenicity and therapeutic effects of a mycobacterium. A dna vaccine was constructed using the mycobacterium marinum ag85a gene, which was isolated and subcloned into a commerciallyavailable eukaryotic expression vector. Vaccination with plasmid dna encoding ag85a from m. A dna vaccine encoding ag85acfp10 fusion protein was.
Immunogenicity and protective efficacy of tuberculosis dna. The bcg and the control groups received bcg and pbs vaccines, respectively. In addition, ag85a dna vaccine prevented the reactivation. Pgacoated complex to pubm, a melanoma dna vaccine, noticeably impeded the growth and metastasis of a melanoma cell line, b16f10 cells. Ag85aesat6 chimeric dna vaccine induces an adverse response. Chapter 3 immunogenic and protective effects of a dna. These results demonstrated that ag85a could strengthen the immune response to iav and s. Research open access a novel dna vaccine expressing the. Immunogenic and protective effects of a dna vaccine for. Dec 19, 20 horse acquired immunity against west nile viruses. A novel dna vaccine expressing the ag85aha2 fusion. This study demonstrated that a dna vaccine encoding the m. A novel dna vaccine expressing the ag85aha2 fusion protein provides protection against influenza a virus and staphylococcus aureus.
Secondary pneumonia due to staphylococcus aureus s. Improved protective efficacy of a speciesspecific dna vaccine. Based on this hypothesis, the mice in this study were vaccinated with a dna vaccine encoding ag85a and tb10. Immunogenicity and protective efficacy of a dna vaccine encoding ag85a from mycobacterium tuberculosis were compared in balbc and c57bl b6 and b10. Isolation, cloning and molecular analysis of ag85a and tb10. Ag85aesat6 chimeric dna vaccine induces an adverse. Evaluation of immune responses to a dna vaccine encoding. May 05, 2020 human trials testing dna vaccines against influenza virus, malaria, hepatitis b virus, hiv, herpes simplex virus, colon cancer, and cutaneous tcell lymphoma are underway and have only just begun. Vaccines are considered to be the most costeffective strategy to. Staphylococcus aureus, influenza a viruse, mycobacterium tuberculosis secreted antigen ag85a, dna vaccine introduction secondary pneumonia due to staphylococcus aureus s. Longcome biological pharmacy, anhui, china or saline cytokine production in vitro. The aim of the present work was to investigate the influence of formulating a dna vaccine encoding ag85a with tmc nanoparticles, on the immune responses generated in mice. Research open access a novel dna vaccine expressing the ag85a. Mechanisms and aspects of relevance for biosafety biosafety first 2007 traavik, t.
Tuberculosis dna vaccine encoding ag85a is immunogenic. Furthermore, the degree of immune responses elicited appeared roughly proportional to the amount of protection conferred. Briefly, peinp coated dna vaccine piresil21ag85aesat6 or pires mock plasmid was prepared by shaking the mixtures of 5 ml of the nanofe 3 o 4glu to 1 ml pei 5. Fingerling hybridstriped bass were obtained from a commercial supplier and housed at the aquatic medicine laboratory of the vmrcvm in. Immunogenicity and therapeutic effects of ag85ab chimeric. Here we have analysed the vaccine potential of a combination of both antigens using plasmid vectors expressing either a fusion protein of both. Host protein glycosylation in nucleic acid vaccines as a. The pei modified dna vaccine was performed as described previously 19, 20. Immunizing the host with a piece of viral dna rather than an antigenic protein fragment of the virus, helps to stimulate. The research presented here successfully created an ag85a dna vaccine that stimulated protective immunity in hybridstriped bass against m. Ag85a gene was firstly amplified by polymerase chain reaction pcr and then cloned into pvax1 to construct the recombinant plasmid pvax1ag85a a.
A novel tuberculosis dna vaccine in an hiv1 p24 protein. Immunogenicity and therapeutic effects of ag85ab chimeric dna. The influence of dna vaccine formulations on immune responses in combination with adjuvants was investigated with the aim to increase cellmediated immunity against plasmid dna pdna encoding mycobacterium tuberculosis antigen 85a. The length of gene sequence encoding for strong immunogen ag85a was 888 bp, and the ag85b gene sequence was 474 bp. Materials and methods plasmid construction and dna vaccine preparation.
The aim of the research was designed a novel dna vaccine encoding the mycobacterium tuberculosis secreted antigen ag85a fused with the influenza a virus iav ha2 protein to provide protection against both influenza and secondary infection with s. An influenza dna vaccine formulated in the lipid compound vaxfectin vical induced protective antibody titers and tcell responses in many subjects. Comparison of bcg primedna booster and rbcg regimens. Jul 11, 2017 antibody production igg and siga anti ag85a was also significantly increased in bronchoalveolar lavage, as well as in the serum of mice. This was achieved by improving the th1type response34. In this study, mycobacterium tuberculosis mtb rv2190c dna vaccine was prepared and its immunogenicity and therapeutic effects were evaluated. Recombinant invasive lactococcus lactis carrying a dna.
May 26, 2006 indeed, vaccination of c57bl6 mice with plasmid dna encoding ag85a and ag85b can stimulate strong humoral and cell. Dou department of pathogenic biology and immunology, medical school, southeast university, nanjing, 29 china. Dna vaccine prepared using recombinant flagellin protein derived from salmonella as mucosal adjuvant for anticaries dna vaccine. Construction of a dna vaccine based on the mycobacterium. It elevated the immunogenicity and protective as well as therapeutic effects of the hsp65dna vaccine against tb in mice. Isolation, cloning and molecular analysis of ag85a and. In order to explore whether ag85a dna vaccine could mount more efficiently protective immune response through new rna sensor and its signal transduction pathway of antigen presentation we designed and synthesized ag85a gene fragment containing multiple points mutations and transfected the gene fragment into the dendritic cell line dc2. Combination chemotherapy is very effective in curing this disease but, unfortunately, the. A multicistronic dna vaccine induces significant protection. Immunizing the host with a piece of viral dna rather than an antigenic protein fragment of the virus, helps to stimulate the generation of cellmediated immunity fig. August 2007,dna vaccination against sclerosis was reported as being effective. A dna vaccine uses a gene from a virus or bacteria to stimulate the immune system. Methods and protocols, third edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of dna vaccines featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on dna vaccine design and enhancement, delivery systems, production, purification, and.
In the study, we developed the dna vaccine encoding fusion protein of antigen 85 a and 6 kda early secretory antigen target of m. Molecular adjuvant ag85a enhances protection against. Aug 01, 2011 an influenza dna vaccine formulated in the lipid compound vaxfectin vical induced protective antibody titers and tcell responses in many subjects. Designing and construction a dna vaccine encoding the fusion. To design a novel multivalent dna vaccine against m. Therapeutic effect of dna vaccines combined with chemotherapy in. A novel dna vaccine vector encoding the mycobacterium tuberculosis secreted antigen ag85a fused with the influenza a virus iav ha2 protein epitopes, pegfpag85asha2 pag85asha2, was designed to provide protection against influenza. Because preparation of mixed dna vaccines is troublesome, not. Protection against mycobacterium tuberculosis challenge in. In light of these findings, it is imperative to investigate the potential impact of mammalian glycosylation on cellmediated immune responses. In balbc mice, gg immunization could induce elevated antibody and cytotoxic t lymphocyte responses with plasmid doses 50fold lower than those.
Briefly, peinp coated dna vaccine piresil21 ag85a esat6 or pires mock plasmid was prepared by shaking the mixtures of 5 ml of the nanofe 3 o 4glu to 1 ml pei 5. Xueqiong wu, yan liang, junxian zhang, li xiao, yourong yang, xuejuan bai, qi yu, zhongming li and ning li. Influence of the formulation characteristics on immune responses. Methods and protocols, third edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of dna vaccines. Dna vaccine expression of il2 and the hsp65 fusion gene was studied. After confirming that they could express successfully in mammalian cells, the novel vaccine and respective controls were used to immunize mice by the intramuscular route before challenge with iav and s. Jan 31, 20 secondary pneumonia due to staphylococcus aureus s. The aim of this study was to design and construct a dna vaccine encoding ag85a and tb10. Vaccines free fulltext ag85a dna vaccine delivery by. Immunogenicity and protective efficacy of a dna vaccine encoding ag85a from mycobacterium tuberculosis were compared in balbc and c57bl b6 and b10 mice immunized by intramuscular i.
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