We show the full front view of the li cor odyssey 9120 infrared imaging system, looking inside the lid, then looking over the software and starting a quickscan. Pair your odyssey clx imager with image studio software for streamlined. Place the blot proteinside down, with wells toward the front, on the odyssey scanning surface. Both membranes were then incubated with donkey antirabbit ir dye 800 licor diluted 1. Download image studio lite software licor biosciences. Licor odyssey clx the new odyssey clx infra red imaging system from licor biosciences improves your quantitative analysis for detecting proteins on membranes, in cells, in tissue, invivo etc. Cor biosciences odyssey, authored by janice thompson, created on april 1, 2009, issued on insert date.
Licor odyssey clx imaging system the lab world group. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel red detection 600 nm are available. This video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. We believe fluxsuite software has great potential, both at home and abroad, for water use efficiency monitoring, targeted irrigation scheduling, and any number of other water andor co 2 management efforts. Free western blot quantification software licor biosciences. Image acquisition and file organization software for use. Image studio lite is western blot analysis software that is compatible with most image files and systems and is available for free download from the li cor website. Powers on, sounds like it turns on however the digital display seems to not. Dna dot blotting and licor odyssey infrared detection. The simple interface puts highquality data at your. If wet, carefully place a silicone mat licor pn 92670001 over the membrane and use. After providing your information, youll get an email with a link to download image studio lite software.
Licor biotechnology instruments, software and reagents, which are based on nearinfrared fluorescent and chemiluminescent detection, are used in a large variety of assays, such as western blot assays. Join us for a live zoom webinar to see how soilfluxpro can simplify management, analysis, and diagnosis of soil gas flux data. Odyssey quickcard starting scans vanderbilt university. The odyssey clx uses the latest version of the licor image studio software. Powers on, sounds like it turns on however the digital display seems to not work and we are unable to properly test. The odyssey system typically uses two ir dyes for simultaneous twocolor detection.
Licor odyssey platform for quantitative western blots. Membranes were scanned by using an odyssey infrared imaging system licor and quantified by using the licor odyssey image studio software version 3. Li cor is excited to roll out fluxsuite software for a global audience, and see how the ec community responds. Odyssey clx application and technical notes show the versatility of the clx. Membranes were then incubated with the appropriate secondary horseradishperoxidaselabelled antibody and developed with the enhanced chemiluminescence method millipore. Used licor odyssey 9120 infrared imaging system 9120 lab. The odyssey clx uses the latest version of the li cor image studio software. Licor biosciences is a global leader in the design, manufacture, and marketing of high quality, innovative instruments, software, reagents, and integrated systems for plant biology, biotechnology. Page 94 if the intensity is set too low, the image may not show any signal even though there is adequate. Odyssey applications featuring irdye infrared dyes infrared dyes are a key performance advantage of the odyssey system. Licor odyssey clx the new odyssey clx infra red imaging system from li cor biosciences improves your quantitative analysis for detecting proteins on membranes, in cells, in tissue, invivo etc. The major advantage with this solution is accurate quantification over a broad dynamic range, up to 6 orders with high sensitivity. Ive had good luck with li cor bb, some success with milk, and generally less success with bsa. The system includes a windows 7 notebook running imaging software and.
Odyssey has a new hard drive installed for optimal life and performance. Licor biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be followed at all times. Image studio, image studio lite, and empiria studio software are used with odyssey imagers. Most blockers are compatible with the odyssey, so id suggest. Odyssey clx infrared scanner vbi core labs vanderbilt. Determining the best blocking agent for each target will provide the best chance for successthe electrophoresis buffers maintain consistent ph levels during dna, rna, and other protein separation. Licor, odyssey, mpx, incell western, and irdye trademarks contained in the software product are trademarks or registered trademarks of licor, inc.
Some quick tips from a li cor application support representative. Licor gmbh germany, austria, switzerland, czech republic, hungary, and slovakia. Licor image studio software for accurate data quantification. Image acquisition and file organization software for use with li cor imaging systems. Li cor laserbased trace gas analyzers are accompanied by a suite of patented technologies that contribute to their robust design and exceptional performance in ch 4 or co 2 measurements, allowing you to be flexible with your research site location. Be aware of the hazards associated with any chemical before you. Thermonuclear 4 abi thermonuclear 1 abi veriti gradient thermonuclear abi 7900ht highthroughput realtime thermocycler, with 96 and 384 well blocks imaging systems biorad chemidoc xrs. When quantitative western blot detection or nearinfrared fluorescent imaging is needed, the bioagilytix team knows the licor platform will deliver the. The licor odyssey western blot delivers quantitative accuracy for target protein detection by using the platforms infrared technology to match or exceed chemiluminescent detection sensitivity.
Targeting the unfolded protein response, xbp1, and the. Odyssey clx infrared imaging system from licor biosciences. The band intensity values were quantified using licor odyssey software licor biosciences, normalized, and plotted lower panel. At licor biosciences, we care about our customers and want them to succeed. Highthroughput detection method for influenza virus. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel. Licor odyssey infrared imaging system w computer and software 6853 r in great cosmetic condition. Although the lite version is free, there is a more comprehensive paid version of the software that aims to easily integrate with the apparatus that licor also sells. Odyssey clx manuals, application notes, software, and webinars. Leveraging this platforms capabilities, bioagilytix is able to perform accurate, reproducible, quantitative western blot assays. Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. The licor odyssey classic is networkconfigured with a standard rj45 communication connection in the rear of the scanner.
Image studio lite is a free software package from li cor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. How the li cor odyssey platform works li cor is an infrared ir laserbased instrumentation that is able to detect western blots without the need for film, a darkroom, or any chemiluminescent substrates. Licor instruments for measuring c 4 photosynthesis the li6400xt and li6800 portable photosynthesis systems are compact, rugged, field portable instruments able to provide researchers. Ive had good luck with licor bb, some success with milk, and generally less success with bsa. Image studio software can be used for quick acquisition and organization of images from most li cor imaging systems, including the odyssey clx. Try free image studio lite software for your western blot quantification. Licor biosciences, odyssey infrared imaging system b446. All of the information comes directly from li cor as they have a truly extensive and easy to access collection of resources available online. Every li cor employee is committed to providing the highest level of customer service and support. John mccord senior sales consultant licor biosciences. Tutorial image files for image studio image studio 5. Constitutive stat3 serine phosphorylation promotes. Licor infrared imaging systems direct infrared detection is available on two different systems from licor biosciences the odyssey infrared imaging.
The simple interface puts highquality data at your fingertips with a few simple clicks and without manual camera adjustments or multiple exposures. For more info, see the software specs you can use image studio lite software for basic western blot analysis, but it doesnt operate li cor imagers or unicorns. Description when your research relies on measurement of protein expression changes, you need data that accurately represents the amount of. Mar 04, 2014 this video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. Li cor biotechnology instruments, software and reagents, which are based on nearinfrared fluorescent and chemiluminescent detection, are used in a large variety of assays, such as western blot assays and cellbased assays, as well as in vivo imaging and dna analysis. Image acquisition and file organization software for use with licor imaging systems. Licor odyssey sa infrared imaging system gmi trusted. Simple, free western blot signal quantification software. Spongebob and his pal patrick need your help collecting bubbles. Image studio lite software is compatible with mac and windows. Odyssey clx imaging system minimize variation and maximize precision with highsensitivity nir fluorescent detection optics engineered to enable definitive analysis of protein. Designed specifically for western blot quantification, image studio lite is.
The licor odyssey clx imager should not be used with any radioactive materials. Licor biosciences pioneering family of irdye infrared dyes are synthesized with reactive functional groups that enable easy covalent coupling to antibodies and other biomolecules. The company is one of the leading manufacturers of nearinfrared and chemiluminescent imaging platforms, analysis software as well as highquality. Li cor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created date. Image studio software is compatible with most licor imaging systems, including the odyssey imaging systems, pearl imaging system, and cdigit blot. The licor odyssey 9120 imager includes two solidstate lasers a 685nm and 785nm that work together to bring a resolution of 237m. Licor presets membrane dna gel protein gel microplate resolution quality. Does anybody have experience with blocking reagents. Get truly quantitative data with the odyssey clx nearinfrared fluorescence. Researchers have already downloaded this software over 80000 times. Be aware of the hazards associated with any chemical before you begin work. Licor biosciences, a 44year old biotech hardware, software, and consumables company, grew out of an agronomy project, the development of light measurement devices for the study of photosynthesis. Molecular imaging on the licor odyssey imaging system.
The li cor odyssey fc imaging system is easy to use and the operation is selfexplanatory. Licor licor odyssey 9120 infrared imaging system imager. This can be efortful because doing this by hand takes some advanced knowledge related to windows program uninstallation. Li cor biosciences, a 44year old biotech hardware, software, and consumables company, grew out of an agronomy project, the development of light measurement devices for the study of photosynthesis. C4 photosynthesis application page licor environmental. Image studio software can be used for quick acquisition and organization of images from most licor imaging systems, including the odyssey clx. The licor odyssey fc imaging system is easy to use and the operation is selfexplanatory. Image studio is used for pearl imagers and cdigit blot scanner. Find detailed information for all software versions.
By using nir fluorescence detection, this imaging system greatly simplifies your western blot process by eliminating the need to incubate your substrate or wait for film. Quantify whole plate or selected wells within plate using licor odyssey software. The li cor odyssey classic is networkconfigured with a standard rj45 communication connection in the rear of the scanner. The only western blot analysis software that guides you from validation through statistical analysis for reliable results. Please visit the main page of odyssey software on software informer. Licor provides the complete solution for quantitative western blots with its data integrity bundle that includes standardized protocols, robust detection, validated reagents, online training, secure data analysis software, and ondemand support.
Licor odyssey infrared imaging system w computer and. If the software product is labeled as an upgrade, you must be properly licensed to use a product identified by li cor as being eligible for the upgrade in order to use the software product. A software product labeled as an upgrade replaces andor supplements the product that formed the basis for your eligibility for the upgrade. Try empiria studio software today and enhance your odyssey workflow with the only western blot analysis software. The cdb equipment core has one additional odyssey clx in mrb3. Odyssey clx infrared imaging system operators manual.
The odyssey clx and odyssey fc imaging systems offer options for nearinfrared and. Twocolor detection adds many new detection capabilities. Li cor provides environmental instruments for eddy covariance. We are a trusted partner, providing training workshops, webinars, videos, printed resources, and amazing technical support. The licor odyssey clx imaging system gives your lab clear sharp digital results in as little as 6 minutes. Tutorial videos and documents help you make the most of your image studio.
Nov 09, 2016 pictured here is james, one of our sr. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual. Metmab, the onearmed 5d5 anticmet antibody, inhibits. Blank negative control wells can be set using the licor odyssey software. Licor introduces the odyssey clx infrared imaging system. The hepatocyte growth factor hgf and its receptor, cmet, have been implicated in driving proliferation, invasion, and poor prognosis in pancreatic cancer. If the software product is labeled as an upgrade, you must be properly licensed to use a product identified by licor as being eligible for the upgrade in order to use the software product. The system includes a windows 7 notebook running imaging software and has been calibrated to factory specification.
Nlrp3 inflammasome as prognostic factor and therapeutic. For example, two different antibodies can be used as probes. Image studio lite is used for basic western blot analysis, but does not operate your li cor instrument. This can be efortful because doing this by hand takes. Below, you will find helpful pdfs for the frequent odyssey users.
Manuals, software information, and webinars are also listed. The instrument is top notch, software is powerful and easy to use for western analysis, and the customer support is excellent. Relative quantification was performed using glycerinaldehyde. By using nir fluorescence detection, this imaging system greatly simplifies your western blot process. The system comes with builtin slicon avalanche photodiode. Electronic technicians, working on an odyssey clx, our nearinfrared molecular imaging platform. The li cor odyssey clx imaging system gives your lab clear sharp digital results in as little as 6 minutes. Licor odyssey clx the odyssey clx combines precision laser illumination, large scan area, and high image resolution to deliver accurate, replicable data capture. When installing the odyssey software on a computer that has the older odyssey software installed, the installation application needs to be run once to remove the older.
Image studio software is compatible with most licor imaging systems, including the odyssey imaging systems, pearl imaging system, and cdigit blot scanner, and can be installed on both windows and mac operating systems. Accurate, replicable western blots with the odyssey clx licor. Licor biosciences pioneering family of irdye infrared dyes are synthesized. For more than 30 years, li cor infrared gas analyzers have been used and trusted by researchers and networks around the world.
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